It highlighted in particular a regulation that came into effect in September, under which Internet users who knowingly make or share information considered defamatory or false could face up to three years' jail time in China.
"The implementation of this newly promulgated judicial interpretation may have a significant and adverse effect on the traffic of our platform and discourage the creation of user generated content," the company said in its filing.
It also warned about potential government intervention in its encryption tools and software - used to prevent spying while safeguarding the privacy of users and their posts.
Weibo said that Beijing requires all "cipher code" products to be registered with the government, though it was unclear whether this applied to social media encryption.
"Because these regulations do not specify what constitutes a cipher code product, we are unsure as to whether or how they apply to us and the encryption software we utilize," the company said in its prospectus.
Come one, come all
Still, U.S. investors have long shown an appetite for Chinese companies' stock, hoping to share in some of the spoils of the world's fastest-growing major economy.
U.S. markets may see more IPOs from Chinese corporations in 2014 than in any year since 2010.
Weibo's revenues leapt almost three-fold to $188.3 million in 2013, from $65.9 million in 2012. And its net loss shrank to $38.1 million in 2013 from $102.5 million the previous year.
But its user growth is at risk of tailing off after three years of explosive expansion, as newer messaging apps such as Tencent Holdings Ltd's WeChat draw users away.
(Read more: China's Baidu fourth quarter revenue tops forecast)