"This deal is about plugging that gap. Consumers told us they wanted more apps," Barnes & Noble CEO William Lynch told Reuters.
Nook HD and Nook HD devices sold in stores as of Friday will already have Google Play installed. Existing customers will be able to download it at Nook's online store and via an automatic over-the-air update.
The tablets will also have Google services like Gmail, YouTube, Google Maps, and the Chrome web browser.
(Read More: Forget the Phone or Tablet: Get Ready for the 'Phablet')
"It's a positive product enhancement- adding the android features is a great step. It makes this device competitive," said Sarah Rotman Epps, a senior analyst with Forrester Research, who nonetheless said the move was not a game changer in the fight against large tech giants whose devices have more visibility.
Google Play is expanding its offering quickly: in the last year, it has rapidly closed the gap with Apple's market-dominating apps store in terms of sheer quantity, with both expected to hit 1 million soon.
The collaboration with Google comes as Barnes & Noble is seeking to reignite customer interest in its Nook, the core part of its Nook Media unit. The bookseller is considering spinning off the Nook Media unit and has already attracted investments from Microsoft and Pearson.
(Read More: Google Launching a Cheaper, More Powerful Tablet in July)
The Nook, launched in 2009 to compete with the Kindle, has been the cornerstone of Barnes & Noble's strategy to benefit from the shift by many readers to digital books.
The retailer sold fewer devices during the crucial holiday season, with revenue in the overall Nook business including content falling 12.6 percent.