1-Stop information is posted in six languages in the corridor leading to the Customs and Border Protection (CBP) passport control area, and instructs those eligible to enter designated lanes.
Travelers are processed at a booth with a CBP officer, and then move on to an express corridor that bypasses baggage claim and CBP exit control inspection areas.
(Read More: United Offers First-Class Care for Animals at Chicago O'Hare)
"This initiative will greatly enhance the experience for travelers entering the U.S. at O'Hare by creating a faster, more efficient process for passengers who are traveling light, and by helping to reduce wait times for all other arriving passengers," said Rosemarie Andolino, commissioner for the Chicago Department of Aviation, in a statement.
(Read More: The Most and Least Stressful US Airports)
O'Hare also will be the first U.S. airport to implement Automated Passport Control (APC), a program to help U.S. passport holders move more quickly through customs by entering information at a self-service kiosk. APC is scheduled to begin this summer.