Last week, Goldman and the LME were named as defendants in a separate Detroit lawsuit.
The latest case, filed by Florida-based aluminium user Master Screens and an individual plaintiff, expands the geography and number of companies being sued.
The companies could face fines if the lawsuits are successful.
Warehouse owners and outgoing LME CEO Martin Abbott have said the complaints over long lines at warehouses are unjustified, arguing there is no shortage of metal.
Goldman Sachs and JPMorgan dismissed the Florida suit. Goldman tried last week to diffuse the criticism over warehousing by offering immediate access to aluminium for end users holding metal at its Metro warehouses.
(Read more: Goldman's aluminium offer may soothe but leave costs high)
"We believe this suit is without merit and we intend to vigorously contest it. We also note that aluminium prices are down 40 percent from their peak in 2006," a Goldman Sachs spokesman said.
"There are no queues at our warehouses. We believe this suit has no merit," a JPMorgan spokesman said.
Hong Kong Exchanges and Clearing Limited, owner of the LME, also said both lawsuits were without merit and the LME would contest them vigorously. It added that it had not been served with either of the suits.
A Glencore spokesman declined to comment.
The Florida lawsuit alleges "manipulation of the aluminium market through supply price fixing," among other practices.
The lead plaintiff in the lawsuit, which is seeking class action status and was filed on Aug. 6 in the U.S. District Court for the Northern District of Florida, is Master Screens Inc. and Daniel Price Bart, described in the filing as "a purchaser of beverages sold in aluminium cans."