The performance did not air nationally on the program, but a small portion of it was captured by Today.com associate editor and Orange Room digital producer Matt Murray and posted on Instagram.
Warren Buffett has brought out his uke before, often performing at the annual Berkshire Hathaway shareholders meeting in Omaha.
In January 2012, a short clip of him playing and singing "I've Been Working on the Railroad" appeared on Chinese television to celebrate the Lunar New Year.
Six months later, he did a duet of "The Glory of Love" with Jon Bon Jovi at the Forbes 400 Summit on Philanthropy.
And if you're wondering, whether Warren Buffett is related to musician Jimmy Buffett, DNA analysis has determined they are not.
—By CNBC's Alex Crippen. Follow him on Twitter @alexcrippen.