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WRAPUP 5-Mediators held in Ukraine as U.S. readies new Russia sanctions

(Adds quote on mediators, colour from Slaviansk, changes dateline)

* Separatists seize international mediators

* Lavrov says U.S. "distorting" Geneva agreement

* S&P cuts Russia's rating, central bank raises interest rate

SLAVIANSK, Ukraine/MOSCOW, April 25 (Reuters) - The United States said on Friday it was prepared to impose further sanctions on Russia over its actions in Ukraine, where armed pro-Moscow separatists seized a bus carrying international mediators, escalating the two-month-old crisis.

The separatist self-declared mayor of Slaviansk told Reuters the mediators were being held because they were believed to have a spy amongst them from the pro-Western government in Kiev.

"People who come here as observers bringing with them a real spy: it's not appropriate," Vyacheslav Ponomaryov said in front of the seized security service building where, according to the Ukrainian government, the observers were being detained.

Moscow denies allegations it is directing the separatists, who have taken control of large parts of eastern Ukraine over the past three weeks.

But the White House said U.S. President Barack Obama and European allies all felt that Russia had escalated tension in the region, where the rebels have refused to leave official buildings they have occupied.

Britain and Germany agreed further sanctions were in order, building on targeted U.S. and European sanctions against Russian and Ukrainian individuals following Russia's annexation of Crimea. But none of the three countries gave any details of what the sanctions might be, or when they might be enacted.

The standoff has already led to heavy capital flight from Russia, prompting credit rating agency Standard & Poor's to cut the country's ratings on Friday. That forced the central bank to raise its key interest rate to reverse a drop in the rouble.

The detention of the mediators, who are working for the Vienna-based Organization for Security and Cooperation in Europe (OSCE), will heighten Western unease about lawlessness and arbitrary rule in separatist-held parts of eastern Ukraine.

"Extremely concerned with OSCE inspectors being abducted in Eastern Ukraine. Including one Swede. They must be released immediately," Swedish Foreign Minister Carl Bildt wrote on his Twitter account.

MILITARY OPERATION

Ukraine sent in troops to try dislodge the separatists for the first time on Thursday, killing up to five rebels around Slaviansk in what it said was a response to the kidnapping and torture of a politician found dead on Saturday.

Russia's foreign minister, Sergei Lavrov, accused authorities in Kiev of waging "war on their own people".

"This is a bloody crime, and those who pushed the army to do that will pay, I am sure, and will face justice," Lavrov said.

The Kremlin says it has the right to defend Russian-speakers anywhere if they are under threat and has deployed extra troops on the border with Ukraine, which NATO says number up to 40,000.

They began military exercises on Thursday and Ukraine said they had approached to within 1 km (1,100 yards) of its border and that it would treat any incursion as an invasion.

Ukrainian special forces launched a second phase of their operation on Friday by mounting a full blockade of Slaviansk, the rebels' military stronghold, a presidential official said.

One of its military helicopters was hit by rocket fire and exploded while on the ground at an airport near the city, the Defence Ministry said.

"TRUTH WILL WIN"

Police were carrying out stringent checks on vehicles on the outskirts of Slaviansk, but did not seem to be preventing movement. In the main square, people strolled around on Friday evening and parents watched their children play.

"We have heard they are surrounding us but there is nothing to be afraid of. The truth will win," shop employee Tetyana, who declined to give her surname, said.

Pro-Western leaders in Kiev, who took power in February after Moscow-ally President Viktor Yanukovich fled following mass protests against him, say they fear Russia will try to take over eastern Ukraine following its annexation of Crimea.

Russian troops seized Ukraine's Crimean peninsula on the Black Sea soon after Yanukovich fled. Moscow denies interfering in eastern Ukraine, as it did in Crimea before admitting its forces had gone in.

The White House statement came after Obama pressed four European leaders on the need for more robust action against Russia. Europe is reluctant to impose tough sanctions due to its reliance on Russian gas and trade ties with Moscow.

"The president noted that the United States is prepared to impose targeted sanctions to respond to Russia's latest actions," it said.

"The leaders agreed to work closely together, and through the G7 and European Union, to coordinate additional steps to impose costs on Russia."

Britain agreed the current sanctions would need to be extended given what it said was Russia's refusal to support an international peace agreement that it signed up to last week under which all sides agreed to work to disarm illegal groups.

German Chancellor Angela Merkel said the leaders would have to react. "Because of the lack of progress we will have to contemplate further sanctions," she said before the call.

France said sanctions had been discussed and the leaders had called for a "speedy reaction" to the crisis from leading industrial nations. The president's office said later it expected a G7 statement in the next few days. Italy said the leaders had agreed on the situation but gave no details.

"The leaders underscored that Russia could still choose a peaceful resolution to the crisis, including by implementing the Geneva accord," the White House statement said.

'ONE-SIDED DEMANDS'

Lavrov said Moscow was committed to implementing the Geneva agreement but accused Washington of distorting it with "one-sided demands". However, Russia's Defence Ministry said it was ready for "unbiased and constructive" talks with the United States to stabilise the situation.

U.S. Secretary of State John Kerry said Russia was using propaganda to hide what it was trying to do in eastern Ukraine - destabilise the region and undermine next month's Ukrainian presidential elections - and decried its "threatening movement" of troops up to Ukraine's border.

Russian President Vladimir Putin has scoffed at the sanctions so far imposed, which have been limited to travel bans and overseas assets freezes on individuals.

Ukraine's Prime Minister Arseny Yatseniuk said Russia wanted to start World War Three by occupying the country and creating a conflict that would spread to the rest of Europe.

Seven people were injured overnight at a pro-Ukrainian checkpoint near the Black Sea port of Odessa when an explosive device blew up, police said on Friday.

Residents have built several such checkpoints near the town aimed at stopping pro-Russian separatists entering from Moldova's breakaway territory of Transdniestria.

NATO warned last month of a possible Russian military grab for Transdniestria following its annexation of Crimea.

(Additional reporting by Roberta Rampton and Arshad Mohammed in Washington, Maria Tsvetkova in Donetsk, Alexei Anishchuk, Lidia Kelly and Oksana Kobzeva in Moscow, Alastair Macdonald and Pavel Polityuk in Kiev, Alexandria Sage in Paris, James Mackenzie in Rome and Erik Kirschbaum in Berlin; writing by Will Waterman and Philippa Fletcher; editing by Mark Trevelyan)