Investors also want to see that Twitter ads are working.
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On the heels of the Super Bowl, which was the largest Twitter event ever (24.9 million tweets related to the game sent during a four-hour period), we can expect CEO Dick Costoloto talk about Twitter as the "second screen," a key companion to TV ad purchases. Will he reveal some revenue numbers from the Super Bowl or an indication of how the company can spin unprecedented advertiser and brand participation on Twitter into more ad dollars?
On the earnings call we can expect plenty of questions about new ways Twitter will make money—how will it build on mobile ad start-up MoPub it purchased, perhaps to build a mobile ad network? And what are plans for social, mobile commerce in light of reports that Twitter commerce chief Nathan Hubbard is working out a deal with mobile payments company Stripe.
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—By CNBC's Julia Boorstin. Follow her on Twitter @JBoorstin.