The Duke said he was not afraid of any unwanted media attention after he created the account, as the move was more about "what was right".
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"If you're trying to encourage young people to be enterprising and think about entrepreneurship, and think about these things, how are the young communicating? They're communicating on Facebook, Twitter, LinkedIn," the Duke told Tania Bryer, host of CNBC Meets.
The Duke, along with the Nominet Trust launched a charity called the iDEA (inspiring Digital Enterprise Award) earlier this year to help young people build their digital skills and test business ideas.
"It (the Twitter feed) is far less about me personally, it's far more about the Office and what we're doing. We've probably taken it to what I would describe as a slightly more commercial level than other members of the royal family," he said.
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Speaking on how he is portrayed by the media, the Prince said the press has a "100 percent right" to criticize him.
"I think that you live with the rough and you live with the smooth. We live in a society where there is freedom of speech, everybody's entitled to their opinion one way or the other. But I know the work that I'm doing is for the benefit, or for the larger benefit of young people, the U.K., and I have I have a level of duty and responsibility," he told CNBC's Tania Bryer.