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"We are disappointed and increasingly concerned by the recent incidents that have overshadowed this NFL season, " the NFL's official beer sponsor said in a statement. "We are not yet satisfied with the league's handling of behaviors that so clearly go against our own company culture and moral code. We have shared our concerns and expectations with the league."
According to consultants IEG LLC, Anheuser-Busch sponsors some 88 percent of the NFL's teams, making it the second-biggest sponsor in the league behind only Gatorade.
The NFL, in a statement, reportedly acknowledged Anheuser's comments.
"We understand. We are taking action and there will be much more to come," the league said, in a statement.
Campbell Soup executives also say they are also watching the NFL's investigation closely.
"Upon completion of the investigation, we expect the NFL to take appropriate action," the company said in a statement. "We have shared our views with the NFL."
Two prominent incidents in recent days have cast a cloud over the professional game.
The Baltimore Ravens released star running back Ray Rice after video emerged of him punching his then-fiancee, while Minnesota Vikings star Adrian Peterson was indicted for allegedly beating his child.
On Tuesday evening, Castrol announced its decision to end its relationship with Peterson. The Vikings also lost a key sponsor this week over the Peterson incident, with Radisson hotels suspending its support of the team.
Despite those episodes, and growing calls for commissioner Roger Goodell to resign, branding experts they expect the game to remain resilient.
—CNBC's Katie Little contributed reporting.