On the other side of the debate, while London has pledged to give Scotland extra powers if it stays in the U.K., there is still a lack of details about what that added authority would be, Hunter said.
Read MoreMarkets await Scottish vote—'be very much concerned'
To help citizens become more informed about what's at stake, Hunter's foundation has published an e-book called "Scotland's Decision," which tackles 16 questions voters should consider before making a decision.
"This is an important question for Scotland, probably the biggest political decision we'll make in my lifetime if not for the next 300 years. Therefore, I knew it was crucial that we didn't leave it to the politicians alone to inform us."
Hunter has already voted on the issue in a mail-in ballot, but he would not divulge whether it was yes or no.
Read MoreDavid Beckham backs No vote: Will Scotland be swayed?
However, Hunter tipped his hand on two important issues.
"Staying with the pound sterling in its current form is a big plus for business. We know the risk. We can quantify the risk. We can manage the risk," he said.
A "yes" vote is "moving a bit into the unknown and therefore the unknown ups our risk, ups our cost."
There is also uncertainty surrounding what will happen with the financial institutions based in Scotland.
If Scotland votes to break away from the U.K., Royal Bank of Scotland and Lloyds have said they'll move to England. Others have said they would make decisions after the vote.
Read More RBS, Lloyds: Just saying no to 'yes' in Scotland
"Financial services is a really important industry," Hunter said. "I would want to do everything in our power to keep and let the financial services industry flourish."
"I don't know what it means for jobs but it's just more uncertainty," he added. "I'm against uncertainty."
—By CNBC's Michelle Fox