Time-Lapse Video of Zebrafish "Inner Ear" Development Wins First Place in Fourth Annual Nikon Small World in Motion Competition

MELVILLE, N.Y., April 27, 2015 (GLOBE NEWSWIRE) -- Nikon Instruments Inc. is pleased to announce the winners of the fourth annual Nikon Small World in Motion Photomicrography Competition. First place for the 2014 competition is awarded to Dr. Mariana Muzzopappa (of the Institute for Research in Biomedicine Barcelona) and Jim Swoger (of the Center for Genomic Regulation, Barcelona), for their stunning capture of the development of a zebrafish lateral line – a process that could provide insight into curing deafness in humans.

The mesmerizing first-place video demonstrates the development of the zebrafish lateral line, a sensory organ analogous to the inner ear of humans, that in fish senses movements in the surrounding water. Muzzopappa and Swoger captured this time-lapse footage by using a combination of transgenes in the fish to label undifferentiated and support cells, and track hair cell differentiation throughout a 36-hour timeframe. The result is an examination of organ development that is stunning not only in its visual effect, but the potential it represents for scientists to one-day replicate the process to counter hearing loss in humans.

Dr. Douglas Clark of Paedia Corporation took second place with a time-lapse look at crystals forming in a single drop of a saturated solution of caffeine in water. This 20-minute process is compressed into just 40 seconds in the video, revealing the beautifully chaotic formation of linear crystalline segments in a constrained space. Clark used polarized diascopic illumination to reveal the rainbow crystals that formed in the solution, a dynamic return to equilibrium from a liquid state.

Finally, Dr. John Hart, professor emeritus of the University of Colorado, Boulder, earned third place for his video of volatile oil film on a water surface. As a professor of atmospheric and oceanic sciences for many years, Hart aimed to capture the small-scale dynamics of evaporation, instability, and coalescence within the oil film that are important to the longevity of fuel spills. Hart also revealed an unexpected beauty, using reflected light differential interference contrast (DIC) techniques to capture the vibrant, almost-shimmering colors.

"The capability to capture and share the movement or development of a specimen under the microscope clearly represents one of the greater advancements in the tools available to the scientific community in recent years – and we are honored to shine a light on some of the best examples each year," said Eric Flem, Communications and CRM manager, for Nikon Instruments. "We are continually amazed that this equipment is involved not only in doing the cutting edge science, but also enabling us all to witness it firsthand. As the deadline for this year's competition approaches, we hope that these winning videos will inspire other scientists and science-enthusiasts to share the beauty and motion they capture under a microscope as well."

The 2014 Nikon Small World in Motion completion was judged by Paul Maddox, Assistant Professor of Biology at the University of North Carolina Chapel Hill.

The deadline for submissions to the 2015 Nikon Small World in Motion competition is April 30th, 2015. Submissions can be sent via www.nikonsmallworld.com.

For additional information, please visit www.nikonsmallworld.com, or follow the conversation on Facebook and Twitter @NikonSmallWorld.


The following are the Top Three Winners and Honorable Mentions for Nikon Small World in Motion 2014. The full gallery of winning videos can be viewed at www.nikonsmallworld.com.

1st Place
Dr. Mariana Muzzopappa with Jim Swoger
Institute for Research in Biomedicine Barcelona
Barcelona, Spain
The development of the zebrafish lateral line, the organ that senses water movements in the fish
4D SPIM (Selective Plane Illumination Microscopy)

2nd Place
Dr. Douglas Clark
Paedia Corporation
San Francisco, California, USA
Time-lapse of caffeine crystallization
Polarized light

3rd Place
Dr. John Hart
Dept. Atmospheric and Oceanic Sci, Univ. Colorado
Boulder, Colorado, USA
Oil film floating on water
Reflected Light Differential Interference Contrast (Nomarski)


Dr. Nancy Costigliola-Tarsky
Harvard Medical School
Brookline, Massachusetts, USA
Vimentin (a protein) undergoing mitosis

Mr. Ralph Grimm
Jimboomba, Queensland, Australia
Rotifer (microscopic water creature)
Differential Interference Contrast

Mr. Gerd A. Günther
Düsseldorf, Germany
Sulfur crystals
Brightfield, Polarized Light

Mr. Elliott Hagedorn
Boston Children's Hospital
Boston, Massachusetts, USA
Time-lapse of a 2 day old zebrafish embryo with green erythrocytes and red blood vessels

Dr. Nils Lindstrom
The Roslin Institute
Edinburgh, Scotland
Developing mouse embryonic kidneys

Dr. Jeremy Logue
National Institutes of Health
Bethesda, Maryland, USA
Human melanoma (cancer) cells blebbing (distorting)

Ms. Xi Lu
University of Maryland
Washington, D.C., USA
2x, 6x

Mr. Pushkar Paranjpe
Post Doctoral Fellow
Bangalore, Karnataka, India
Tracking the locomotion of a fruit fly
200 Frames/s video acquired using an AVT Pike camera

Dr. Masha Prager-Khoutorsky
Centre for Research in Neuroscience
Montreal, Quebec, Canada
Aplysia (sea slug) neuron

Mr. Alex Ritter with Dr. Bi-Chang Chen (Academia Sinica, Taipei, Taiwan), Dr. Wesley Legant (Janelia Farm Research Campus, Ashburn, VA), Dr. Liang Gao (Stony Brook University, Stony Brook, NY)
National Institutes of Health
Washington, D.C., USA
Killer T cell engaging a cancer cell
Light Sheet Microscopy

Ms. Ashley Smith
CFD Research Corporation
Huntsville, Alabama, USA
Leukocyte Inflammation Response

Mr. Wim van Egmond
Micropolitan MuseumBerkel en Rodenrijs, Netherlands
Lacrymaria olor (ciliate)
Differential Interference Contrast

Mr. Shaohe Wang
University of California, San Diego
La Jolla, California, USA
C. elegans (nematode) cell division

Mr. Kevin Yehl
Emory University
Atlanta, Georgia, USA
DNA micro-machine rolling across a fluorescently labeled RNA surface


The Nikon Small World Photomicrography Competition is open to anyone with an interest in photography. Participants may submit their images in traditional 35mm format, or upload digital images directly at www.nikonsmallworld.com. For additional information, contact Nikon Small World, Nikon Instruments Inc., 1300 Walt Whitman Road, Melville, NY 11747, USA or phone (631) 547-4200.


Nikon Instruments Inc. is a world leader in the development and manufacture of optical and digital imaging technology for biomedical applications. Now in its 97th year, Nikon provides complete optical systems that offer optimal versatility, performance and productivity. Cutting-edge instruments include microscopes, digital imaging products and software. Nikon Instruments is one of the microscopy and digital imaging arms of Nikon Inc., the world leader in digital imaging, precision optics and photo imaging technology. For more information, visit www.nikoninstruments.com. Product-related inquiries may be directed to Nikon Instruments at 800-52-NIKON.

CONTACT: Media Contact: Trisha Bruynell Peppercomm 212-931-6129 tbruynell@peppercomm.com

Source: Nikon Instruments