Since the May 7 election, many businesses have urged the government to call the referendum sooner rather than later in order to end the uncertainty that could hit confidence and the economy.
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Bank of England Governor, Mark Carney, added his voice to the debate last week, saying it was "in the interests of everybody" to resolve the issue of the U.K.'s EU membership.
As one of the world's biggest financial centres, any changes to how major banks operate in the U.K.'s capital city could have far-reaching consequences.
Deutsche Bank, the euro zone's second-largest bank in terms of assets, has just under 9,000 employees in the U.K. Its presence in the country dates back to 1873.
Robert Noel, CEO of Land Securities, a commercial property firm that has its headquarters in central London, said he did not believe that businesses would leave the city if the U.K. leaves the EU– an event referred to as "Brexit."
"London is the global financial centre. No one can challenge it," Noel told CNBC Europe's "Squawk Box" on Tuesday, in answer to a question about whether he was worried about London losing its status.
"We don't like the uncertainty of potentially coming out of the EU, and as soon as that uncertainty is cleared up, we can get on with our lives. These people are not going to leave our shores in droves," he added.