The trailer features Lighting McQueen spinning out of control during a race before flipping into a fiery cloud of wreckage. The screen fades to black and the words "From this moment, everything will change" appear.
Twitter users were quick to comment about the teaser, calling it "sad," "emotional" and "dark." Others, began sharing their theories for what the trailer means for the franchise.
"Now that's how you shake things up," Paul Dergarabedia, senior media analyst for comScore, told CNBC.
"This trailer says this is not your typical 'Cars' movie and literally puts a serious spin on what has traditionally been a very bright and cheery franchise."
Disney did not immediately respond to CNBC's request for comment.
Pixar, which merged with Disney in 2006, has released a new film about once a year since 1995. The animation company, which works independently from Disney Animation, has garnered great success in the industry with films like "Finding Dory," "Inside Out" and its "Toy Story" franchise.
Its "Cars" franchise, however, has not preformed as well for the company. While, the two films have garnered a significant amount at the global box office, "Cars" and "Cars 2" are middle of the pack when it comes to Pixar's box office successes.
"Cars" earned $462 million at the box office in 2006 and "Cars 2" brought in about $562 million. Here's how those figures compare to other Pixar releases: