Mary Grace Gardner, a career strategist at The Young Professionista, tells Glassdoor that there are many reasons a hiring manager may pose this question.
"If an employer wants to understand what makes you uncomfortable, they want to better understand your limits, both in terms of how far you are willing to push yourself to grow as well as your ethical boundaries," she says.
No matter what your answer, it's important to avoid saying that an essential function of the position you are applying for makes you uncomfortable. "You don't want to highlight anything that's a key part of the role," says career coach Angela Copeland. "For example, if you're in sales, you don't want to mention that talking to people you don't know makes you uncomfortable."
Instead, Copeland suggests using this question as an opportunity to communicate your strong ethical values.
"Select something that will reflect positively on you — perhaps it makes you uncomfortable when a coworker behaves in an unethical way at work," she says.
By taking this approach, you can make sure you nail your interview and feel comfortable with your performance.
Like this story? Like CNBC Make It on Facebook
How to answer the interview question, 'How would you fire someone?'
How to answer the interview question, 'Would you work more than 40 hours a week?'
How to answer the interview question, 'What are some of your leadership experiences?'