The settlement would make her the fourth-richest woman in the world. She ranks behind Francoise Bettencourt Meyers, Alice Walton and Jacqueline Mars, according to Bloomberg.
According to a filing with the Securities and Exchange Commission, MacKenzie Bezos will retain shares representing about 4 percent of Amazon's outstanding common stock, making her the third-biggest shareholder at the company, behind her ex-husband and Vanguard. He remains the richest person in the world, even after losing $35.6 billion in Amazon stock.
According to the latest proxy filing, Jeff Bezos will remain Amazon's biggest shareholder, with a stake of about 12%.
Amazon's stock was down about 0.4% Thursday afternoon following the news and closed down 0.1%.
In a separate statement, Jeff Bezos shared his gratitude to his ex-wife.
"I'm grateful for her support and for her kindness in this process and am very much looking forward to our new relationship as friends and co-parents," Bezos said.
The announcement clears up some concerns investors had in the days after the couple announced their plans to get a divorce about who would have voting power at Amazon. The Bezoses' split represented a unique situation, according to divorce law experts, since the two were together before Amazon started and before either of them owned such a vast amount of money.
While other founders and CEOs have gotten divorces, most had met their spouses after acquiring their wealth. MacKenzie Bezos, on the other hand, reportedly played a significant role laying Amazon's roots. In an oft-cited origin story, MacKenzie drove the pair from New York to Seattle while Jeff wrote Amazon's business plan. MacKenzie eventually negotiated Amazon's first freight contracts, according to Wired.
Watch: Jeff Bezos 1999 interview on Amazon before the dotcom bubble burst