While Zuckerberg expressed concern for his company's shareholders, he has made it clear in the past that the company's mission does not revolve around them.
"Going public is an important milestone in our history. But here’s the thing: our mission isn’t to be a public company. Our mission is to make the world more open and connected," Zuckerberg said in a speech before he rang the opening bell at the NASDAQ May 18, the day the company went public.
Zuckerberg reiterated Facebook's mission Tuesday when said "When you look back 10, 20 years from now, the legacy of this company should be that we've connected everyone in the world and that everyone can share all the stuff that they want."
Major Facebook investors Peter Thiel and Dustin Moskovitz have both recently sold large quantities of company stock, also stirring concern among investors.
Despite the criticism Facebook has faced regarding its stock price, though, Zuckerberg said it is common for his company to go through waves of varying public opinion.
"There are these times where everyone in the world thinks everything that we are doing is awesome and usually they are too optimistic when they think that...and then there are times when people are super pessimistic," Zuckerberg said. "Personally, I would rather be in the cycle where people underestimate us...I think a bunch of people are." (Read More: Facebook Fundamentals Are Strong: FB Adviser)
Wall Street personality Henry Blodget said he thinks Zuckerberg's comments Tuesday may have captured Wall Street's attention.
“He acknowledged that he’s disappointed in the stock’s decline. He said look, we care about shareholders - that’s something that shareholders want to hear,” Blodget said. (Read More: These Zuckerberg Comments Matter Most: Blodget)
Shares popped over 4 percent in after-hours trading.
Zynga shares also got a little bump after-hours. (Click here for after-hours Facebook and Zynga quotes)