Mark Cuban asks how many employees has Donald Trump turned into millionaires?

Dallas Mavericks owner Mark Cuban went after Donald Trump on Twitter less than 24 hours after the first presidential debate.

Cuban started by taking pride in sharing the proceeds of selling companies with his employees, claiming he has made more than 300 millionaires.

@mcuban: 1) I'm proud of the fact that for each of the companies I sold, I share the proceeds with my employees. I've mad more than 300 millionaires

He continued by asking when Trump has done the same for his employees.

@mcuban: 2) Has Trump rewarded any employees when he has succeeded ? All those supposed billions. Has he made a single employee a millionaire ?

Cuban, who has endorsed Hillary Clinton, then pivoted to criticizing the GOP nominee for not proposing any policy changes to help entrepreneurs.

@mcuban: 3) Has Trump offered a single proposal to reduce the friction of starting a business. @HillaryClinton has

The Dallas Mavericks owner has relentlessly trolled Trump and openly slammed his policy proposals on Twitter.

Trump's campaign did not immediately respond to CNBC's request for comment.

Disclosure: CNBC owns the exclusive off-network cable rights to "Shark Tank," which features Mark Cuban as a judge.