In addition to that, tens of thousands of dollars were spent so that IRS employees could also hear from an "innovation expert" and a "diversity and inclusion expert."
One of the speakers received a $25,000 fee in addition to a $2,500 first-class airline ticket to fly the speaker to the conference, according to the report.
The contracts for the presenters were awarded as sole source contracts, meaning no competition occurred, and taxpayers cannot be assured to have received the best price for the speakers.
Sources told NBC News that the conference was approved by two of the agency's deputy commissioners: Mark Ernst, who left the IRS in 2010, and Steve Miller, now the resigned former acting commissioner.
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The revelations were made as part of the Treasury Department's Inspector General's report on IRS spending for travel and conference that will be released Tuesday afternoon.
According to the report, the IRS spent nearly $50 million on at least 220 conferences for employees between 2010 and 2012.
A particular focus of the investigation was the Anaheim conference, which cost taxpayers $4 million, a statement by the House Oversight and Government Reform Committee said.
The IRS failed to adhere to the standard government practice of negotiating lower room rates, and gave the 2,600 attendees perks like baseball tickets and stays in presidential suites, according to the congressional committee.
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On Friday, another video was released showing about a dozen IRS employees practicing the "Cupid Shuffle" dance. That recording was produced to be shown at the end of the 2010 conference, and comes on the heels of two other wacky training videos that surfaced in March, parodying "Gilligan's Island" and "Star Trek."
Combined, the total cost of the video parodies was more $60,000, the agency said.
The forthcoming report will outline lavish spending comes at the same time the IRS is being scrutinized for targeting conservative groups applying for tax-exempt status and as government agencies, and as other government agencies struggle to cut spending in the wake of Washington budget battles.
Danny Werfel, the new acting commissioner of the IRS, released a statement Friday calling the conference "an unfortunate vestige from a prior era."
Werfel made his first appearance in front of Congress on Monday to address the agency's targeting of conservative groups. He told lawmakers, "My primary mission is to restore that trust."
—By Kelly O'Donnell and Andrew Rafferty of NBC News