Blankfein responded, telling CNBC's "Power Lunch" on the sidelines of Goldman Sachs' Builders + Innovators Summit that it is a "waste of time" to argue against "irrational" comments and that it is a "very extreme moment" in politics.
Last week, Trump alleged at a rally in the swing state Florida that Clinton "meets in secret with international banks to plot the destruction of U.S. sovereignty in order to enrich these global financial powers, her special interest friends and her donors."
"We could parse that clause by clause, but to every clause, the answer is 'no, we weren't doing it.' We weren't meeting in secret and we certainly weren't plotting destruction," Blankfein said with a smile.
The Goldman CEO's comments follow renewed criticism of Clinton and the perception of her cozy relationship with Wall Street, fueled by purportedly hacked emails from Clinton campaign chairman John Podesta released by WikiLeaks.
NBC News has not authenticated any of the alleged emails. The Clinton campaign has not offered authentication of the alleged emails.