McCabe denied that Comey had lost support of the rank-and-file FBI agents. The White House suggested Wednesday that a loss of confidence in Comey among FBI employees contributed to his firing.
"Director Comey enjoyed broad support within the FBI and still does today," McCabe said.
The Trump administration has suggested multiple motives for Comey's termination, including his conduct in the probe into Hillary Clinton's handling of classified information last year and an error in his congressional testimony on the probe last week. However, the timing of the firing — which comes as the bureau is investigating the president's campaign — has raised concerns from lawmakers on both sides of the aisle, including several Senate Republicans.
It has also sparked questions about whether the Trump administration will attempt to derail the probe.
In his letter firing Comey, Trump went out of his way to write that the FBI director told him three times he was not under investigation. Such a statement from Comey would not appear to follow the FBI's protocol, multiple reports said.
Asked Thursday if he knew if Comey told Trump he was not under investigation, McCabe said he could not comment. Later in the hearing, McCabe said he would not give the White House such updates on the investigation.
He called it a "highly significant investigation." The White House has contended that there is nothing underpinning the probe and Americans should move on from it.
McCabe said he was not aware of a reported Comey request for more resources for the probe, adding that he believes it is "adequately" funded.
Democrats have decried the firing and increased their calls for a special prosecutor in the Russia investigation. Most Republican lawmakers, though, have resisted those calls and said that the Senate Intelligence Committee's probe into Russian interference is adequate.
Trump has repeatedly denied that his campaign colluded with Russia, and has even suggested that Moscow may not be responsible for cyberattacks on Democrats. Russian President Vladimir Putin has said Russia did not meddle in the 2016 election, though the consensus among foreign policy experts in the United States and Europe is that Russian routinely interferes with elections in the West.
McCabe, who joined the FBI 20 years ago and became deputy director last year, is respected within the bureau. Comey enjoyed similar popularity among the rank and file of the FBI.