The latest injection brings the startup company's total funding to 5 billion yuan, it said in a statement. It follows an earlier round in December in which Alibaba also participated.
Xiaopeng, which debuted its electric car at CES in Las Vegas this month, is one of several Chinese start-ups looking to speed up development of battery-powered technology and compete with global leaders including Tesla.
China's new energy vehicle sales are expected to grow by 40 percent in 2018, according to an industry body, even as the country's traditional automotive market slowed sharply in 2017.
Xiaopeng says its first vehicle, the 'G3', will hit the market this year.
Alibaba has invested heavily in smart car technology and partnered with a handful of traditional western and Chinese carmakers, including Ford and BMW.
Other investors in the round include IDG Capital as well as previous investors GGV Capital, Morningside Venture Capital and Matrix Partners, Alibaba said on its press site on Sunday.
Russian billionaire investor Yuri Milner is also supporting the initiative, the web site said.