The deal includes Berkshire Hathaway Media Group and The Buffalo News, a paper covering western New York and owned as a separate entity by Berkshire.
Lee said the purchase of Berkshire's newspaper assets will "add significant size and scale" to its operations, lift its portfolio of daily newspapers to 81 from 50 and nearly double its audience.
In a statement released with Lee's announcement, Buffett said: "My partner Charlie Munger and I have known and admired the Lee organization for over 40 years. They have delivered exceptional performance managing BH Media's newspapers and continue to outpace the industry in digital market share and revenue."
"We had zero interest in selling the group to anyone else for one simple reason: We believe that Lee is best positioned to manage through the industry's challenges," he added.
Lee said the acquisition will have an immediate boost to profitability and will cut its leverage ratio — a measure of a business's debt as part of its total capital — before any cost and revenue synergies.
The deal will include local news organizations such as the Omaha World-Herald in Nebraska and the Tulsa World in Oklahoma. Davenport, Iowa-based Lee Enterprises already owns outlets such as the St. Louis Post-Dispatch and The Bismarck Tribune in North Dakota.
"This highly collaborative relationship has driven digital and subscription revenue growth, margin expansion and continued innovation," Lee CEO Kevin Mowbray said in a release.