ESPN informed its staff about the layoffs on its internal website, and encouraged employees to contact their supervisor with any questions.
ESPN's layoffs were first reported on Tuesday by Gawker Media's sports blog, Deadspin.
The cable channel declined to comment beyond saying in a statement that "we are implementing changes across the company to enhance our continued growth while smartly managing costs."
Reuters reported in April that Disney, which owns 80 percent of ESPN, was cutting 150 jobs at its studio and an undisclosed number at its consumer products division. The Burbank, Calif.-based company also laid off about 150 employees at its newly acquired Lucasfilm unit in April.
(Read More: Disney Readies for Layoffs in Wake of Home Entertainment Declines)
In Disney's most recent fiscal half year, ended March 30, its cable operation—which ESPN dominates—accounted for 54.7 percent of overall operating income. Theme parks, its second largest money earner, accounted for just under 20 percent of operating income.
In May, ESPN said it would pay for the rights to U.S. Open Tennis that it did not already have for an amount widely reported to be $825 million. It also said in May that it was starting a new channel focused on the Southeastern Conference, or SEC, that will carry 45 college football games starting in 2014.
Like all sports channels, ESPN been struggling with rising sports programming costs, but it commands the highest affiliate fees paid by cable systems, which are on the rise.
(Read More: As TV Migrates Online, Cable Is Under Pressure to Change)
ESPN receives $5.15 per subscriber per month and is seen in well over 101 million homes, according to the National Cable & Telecommunications Association. Disney's cable unit earnings rose by 8 percent, to $2.7 billion, in the latest fiscal 6-month period, the company said.
DirecTV, Dish Network and Time Warner Cable are just some of the cable and satellite TV operators that have complained about rising sports fees that ESPN and its rivals charge.