Losses in the region came after all three major U.S. indexes closed lower, despite notching gains earlier in the session, as technology stocks weighed on the broader markets. The drop in large cap tech names saw the Nasdaq composite fall 2.93 percent.
Facebook shares tumbled after Bank of America Merrill Lynch cut its price target on the stock for the second time in five days. Other tech favorites, including Amazon, Netflix and Alphabet, also fell sharply.
Apart from tech sector troubles, trade-related issues returned to the spotlight as investors focused on a Bloomberg News report that the Trump administration was considering using an existing emergency law to limit Chinese investment in technologies regarded as sensitive.
While the report wasn't exactly "new news," given the Trump administration's recent move to block a proposed Broadcom-Qualcomm deal on national security concerns, it was "enough for investors to become defensive again," David de Garis, director of economics at National Australia Bank, said in a morning note.
Still, the overnight declines were not indicative of the broader economy, Colin Graham, CIO of multi-asset solutions at Eastspring Investments said.
"I think that the tech stocks had become detached from what's going on in the real economy ... But, for us, the underlying economy is still fine. We've seen some weakness in some of the survey data, but they're coming from very high levels," Graham told CNBC's "Capital Connection."
Stocks in Asia and Europe had bounced in the last session after U.S. and Chinese officials made conciliatory comments on Monday that suggested there could be some positive developments on trade.
In individual stocks, shares of Hong Kong-listed BYD Company sank 10.96 percent by 3:06 p.m. HK/SIN after the company reported Tuesday that its full-year profit declined 19.5 percent. It also said first-quarter net profit was expected to be pressured after a reduction in new energy vehicle subsidies.
Meanwhile, SoftBank Group and Saudi Arabia announced on Wednesday they were developing the world's largest solar power generation project, which is expected to cost $200 billion through 2030. Shares of SoftBank closed down 4.01 percent.
In currencies, the dollar index, which tracks the greenback against a basket of currencies, extended overnight gains to trade at 89.431 at 2:42 p.m. HK/SIN. The index had touched a five-week low in the overnight session.
Against the yen, the dollar firmed to trade at 105.65.
On the commodities front, oil prices extended losses after edging lower overnight. U.S. West Texas Intermediate crude futures slipped 0.93 percent to trade at $64.64 per barrel and Brent crude futures declined 0.83 percent to trade at $69.53.